Concentrations (example)

Please note: All concentrations shown here are exemplatory. They do not reflect real data for the described products. Actual concentrations depend on the production lot of each product and can exclusively be found in the product information leaflet. Please ask for these product information leaflets or download them from the service site of the Chromsystems website.

Substance	Unit	Value Level I	Range Level I	Value Level II	Range Level II	Value Level III	Range Level III
LC-MS/MS
25-OH-Vitamin D3	µg/l nmol/l	16.7 41.7	13.4-20.1 33.4-50.1	38.4 95.8	30.7-46.1 76.6-115	104 260	83.3-125 208-312
25-OH-Vitamin D2	µg/l nmol/l	17.4 42.1	13.9-20.9 33.7-50.5	38.7 93.9	31.0-46.5 75.1-113	94.6 229	75.7-114 183-275
HPLC
25-OH-Vitamin D3	µg/l nmol/l	29.0 72.4	23.2-34.8 57.9-86.9	88.9 222	71.1-107 178-266	----	----
25-OH-Vitamin D2	µg/l nmol/l	27.7 67.1	22.2-33.2 53.7-80.6	84.3 204	67.4-101 163-245	----	----

Instructions

These Chromsystems serum controls are designed to monitor the accuracy and precision of analytical procedures for the quantitative determination of 25-OH-vitamin D3 and 25-OH-vitamin D2 in serum/plasma. The lyophilised control is based on human serum and should be used routinely according to the instructions accompanying the assay procedure being used. While following these instructions the control is to be handled in the same manner as a patient specimen

Reconstitution

Remove the metal seal and rubber stopper from the vial. Add exactly 1.0 or 2.0 ml of double distilled water (possibly HPLC-grade) into the vial. Replace the stopper and allow to sit for 10 to 15 minutes at room temperature. Swirl the vial to dissolve the contents until homogeneity.

Storage conditions

Stored at ≤-18 °C unopened controls are stable until the expiry date which is stated on the vial labels. The reconstituted control can be stored up to one week light protected and tightly capped at +2 to +8 °C. If the controls cannot be analysed within this period, they should be aliquoted and stored frozen at ≤-18 °C (up to 3 months).

Ordering information

LC-MS/MS
0221	MassCheck® 25-OH-Vitamin D3/D2 Serum Control (lyoph.), Bi-Level	2 x 5 x 1 ml
0222	MassCheck® 25-OH-Vitamin D3/D2 Serum Control (lyoph.), Level I	5 x 1 ml
0223	MassCheck® 25-OH-Vitamin D3/D2 Serum Control (lyoph.), Level II	5 x 1 ml
0256	MassCheck® 25-OH-Vitamin D3/D2 Serum Control (lyoph.), Level III	5 x 1 ml

HPLC
0028	25-OH-Vitamin D3/D2 Serum Control (lyoph.), Bi-Level	2 x 5 x 2 ml
0029	25-OH-Vitamin D3/D2 Serum Control (lyoph.), Level I	5 x 2 ml
0030	25-OH-Vitamin D3/D2 Serum Control (lyoph.), Level II	5 x 2 ml